Rabbit anti-Human ADM Polyclonal Antibody | anti-ADM antibody

ADM, ID (ADM, AM, Adrenomedullin, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide) (MaxLight 650)

Cat. Num. AAA6271802

• Gene Names: ADM; AM
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADM; Polyclonal Antibody; ID (ADM; AM; Adrenomedullin; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide) (MaxLight 650); ProAM N-terminal 20 peptide; anti-ADM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADM.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ADM antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 69-96 of human ADM. Species sequence homology: bovine
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse lung tissue lysates (35ug/lane) usingMBS648888. ADM (arrow) was detected usingMBS648888.)

Western Blot (WB)

(Western Blot analysis in MCF-7 whole cell lysate (20ug) using MBS648888 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 20kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis is MDA-MB435 cells using MBS648888 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Testing Data

(Overlay histogram showing A549 cells stained with MBS648888 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by MBS648888 (1:25) for 60 mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 for 40 mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Related Product Information for anti-ADM antibody

ADM, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes.

Product Categories/Family for anti-ADM antibody

Antibodies; Hormones; Steroids

References

1. Kim, S.M., et al. FEBS Lett. 584(1):213-218(2010). 2. Oie, E., et al. Basic Res. Cardiol. 105(1):89-98(2010). 3. Nomura, I., et al. Regul. Pept. 158 (1-3), 127-131 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 765330
• NCBI GeneID: 133
• UniProt Accession #: P35318; Q6FGW2; B2R793; D3DQV3
• Molecular Weight: 20,420 Da
• NCBI Official Full Name: adrenomedullin
• NCBI Official Synonym Full Names: adrenomedullin
• NCBI Official Symbol: ADM
• NCBI Official Synonym Symbols: AM
• NCBI Protein Information: ADM; preproadrenomedullin
• UniProt Protein Name: ADM
• Protein Family: ADM
• UniProt Gene Name: ADM
• UniProt Synonym Gene Names: AM; AM; PAMP; ProAM-N20
• UniProt Entry Name: ADML_HUMAN

NCBI Description

Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element. [provided by RefSeq, Jul 2008]

Uniprot Description

ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; adrenomedullin receptor binding; hormone activity; receptor binding

Biological Process: developmental growth; positive regulation of apoptosis; blood circulation; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; signal transduction; positive regulation of vasodilation; response to insulin stimulus; negative regulation of cell proliferation; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; cell-cell signaling; receptor internalization; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; response to wounding; vasculogenesis; regulation of the force of heart contraction; aging; hormone secretion; organ regeneration; progesterone biosynthetic process; androgen metabolic process; neurite regeneration; positive regulation of heart rate; negative regulation of vasoconstriction; odontogenesis of dentine-containing teeth; response to starvation; positive regulation of angiogenesis; G-protein coupled receptor internalization; neural tube closure; response to hypoxia; negative regulation of vascular permeability; cAMP biosynthetic process; response to cold