Rabbit YMEL1 Polyclonal Antibody | anti-YME1L1 antibody

YMEL1 Antibody (N-term)

Cat. Num. AAA9207100

• Gene Names: YME1L1; FTSH; MEG4; PAMP; YME1L
• Reactivity: Human, hamster (Predicted Reactivity: Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: YMEL1; Polyclonal Antibody; YMEL1 Antibody (N-term); ATP-dependent zinc metalloprotease YME1L1; 3424-; ATP-dependent metalloprotease FtsH1; Meg-4; Presenilin-associated metalloprotease; PAMP; YME1-like protein 1; YME1L1; FTSH1; YME1L; anti-YME1L1 antibody

• Host: Rabbit
• Reactivity: Human, hamster (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This YMEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-219 amino acids from the N-terminal region of human YMEL1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 191-219
• Sequence Length: 683

• Applicable Applications for anti-YME1L1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of YMEL1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of YMEL1 Antibody (N-term) in CHO cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(YMEL1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YMEL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(YMEL1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-YME1L1 antibody

YMEL1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.

Product Categories/Family for anti-YME1L1 antibody

Metabolism; Signal Transduction

References

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Deloukas, P., et al. Nature 429(6990):375-381(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 359718987
• NCBI GeneID: 10730
• NCBI Accession #: NP_001240795.1; NP_055078.1; NP_647473.1
• NCBI GenBank Nucleotide #: NM_001253866.1
• UniProt Accession #: Q96TA2; Q5T8D9; Q9H1Q0; Q9UMR9; B4DNM1; D3DRV8; D3DRV9
• Molecular Weight: 86455
• NCBI Official Full Name: ATP-dependent zinc metalloprotease YME1L1 isoform 4
• NCBI Official Synonym Full Names: YME1-like 1 ATPase
• NCBI Official Symbol: YME1L1
• NCBI Official Synonym Symbols: FTSH; MEG4; PAMP; YME1L
• NCBI Protein Information: ATP-dependent zinc metalloprotease YME1L1
• UniProt Protein Name: ATP-dependent zinc metalloprotease YME1L1
• Protein Family: ATP-dependent zinc metalloprotease
• UniProt Gene Name: YME1L1
• UniProt Synonym Gene Names: FTSH1; YME1L; PAMP
• UniProt Entry Name: YMEL1_HUMAN

NCBI Description

The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

YME1L1: Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: membrane; mitochondrial inner membrane; integral to membrane

Molecular Function: metalloendopeptidase activity; metal ion binding; ATP binding

Biological Process: cell proliferation; mitochondrion organization and biogenesis; misfolded or incompletely synthesized protein catabolic process