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Western Blot (WB) (Western blot analysis of anti-STK29 Pab in HL60 cell line lysate (35ug/lane). STK29(arrow) was detected using the purified Pab.)

Rabbit anti-Human STK29 Polyclonal Antibody | anti-BRSK2 antibody

STK29 Antibody (C-term)

Gene Names
BRSK2; SAD1; SADA; STK29; PEN11B; C11orf7
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STK29; Polyclonal Antibody; STK29 Antibody (C-term); Serine/threonine-protein kinase BRSK2; Brain-selective kinase 2; Brain-specific serine/threonine-protein kinase 2; BR serine/threonine-protein kinase 2; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase SAD-A; BRSK2; C11orf7; PEN11B; SADA; anti-BRSK2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STK29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-736 amino acids from the C-terminal region of human STK29.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
706-736
Sequence Length
736
Applicable Applications for anti-BRSK2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-STK29 Pab in HL60 cell line lysate (35ug/lane). STK29(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-STK29 Pab in HL60 cell line lysate (35ug/lane). STK29(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-BRSK2 antibody
BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C, and CDC25B in an in vitro assay, but a kinase-dead mutant does not. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.
Product Categories/Family for anti-BRSK2 antibody
References
J. Biol. Chem. 279: 31164-31170, 2004.
J. Hum. Genet. 44:1-9(1999).
J. Hum. Genet. 43: 283-284, 1998.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81633
NCBI Official Full Name
serine/threonine-protein kinase BRSK2 isoform 1
NCBI Official Synonym Full Names
BR serine/threonine kinase 2
NCBI Official Symbol
BRSK2
NCBI Official Synonym Symbols
SAD1; SADA; STK29; PEN11B; C11orf7
NCBI Protein Information
serine/threonine-protein kinase BRSK2
UniProt Protein Name
Serine/threonine-protein kinase BRSK2
UniProt Gene Name
BRSK2
UniProt Synonym Gene Names
C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2
UniProt Entry Name
BRSK2_HUMAN

Uniprot Description

BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.26; Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: centrosome; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm

Molecular Function: protein serine/threonine kinase activity; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; mitosis; peptidyl-serine phosphorylation; exocytosis; axonogenesis; cell division; establishment of cell polarity; actin cytoskeleton reorganization; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation

Research Articles on BRSK2

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Product Notes

The BRSK2 brsk2 (Catalog #AAA9206743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 706-736. The STK29 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK29 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the BRSK2 brsk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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