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Western Blot (WB) (All lanes : Anti-Integrin beta 5 Antibody at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 88 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit Integrin beta 5 Polyclonal Antibody | anti-ITGB5 antibody

Integrin beta 5 Antibody

Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Integrin beta 5; Polyclonal Antibody; Integrin beta 5 Antibody; Integrin beta-5; ITGB5; anti-ITGB5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
721-780
Sequence Length
799
Applicable Applications for anti-ITGB5 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human Integrin beta 5
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-Integrin beta 5 Antibody at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 88 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-Integrin beta 5 Antibody at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 88 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ITGB5 antibody
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.
Product Categories/Family for anti-ITGB5 antibody
References
Ramaswamy H.,et al.EMBO J. 9:1561-1568(1990).
Suzuki S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5354-5358(1990).
McLean J.W.,et al.J. Biol. Chem. 265:17126-17131(1990).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zhang H.,et al.Nat. Cell Biol. 6:523-531(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88054
NCBI Official Full Name
integrin beta-5
NCBI Official Synonym Full Names
integrin, beta 5
NCBI Official Symbol
ITGB5
NCBI Protein Information
integrin beta-5
UniProt Protein Name
Integrin beta-5
Protein Family
UniProt Gene Name
ITGB5
UniProt Entry Name
ITB5_HUMAN

Uniprot Description

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: cell surface; focal adhesion; leading edge; plasma membrane; phagocytic vesicle; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; extracellular matrix organization and biogenesis; muscle contraction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I

Research Articles on ITGB5

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Product Notes

The ITGB5 itgb5 (Catalog #AAA9205739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 721-780. The Integrin beta 5 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). WB~~ 1:1000. Researchers should empirically determine the suitability of the ITGB5 itgb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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