Western Blot (WB)
(NR1H4 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR1H4 antibody detected the NR1H4 protein (arrow).)
Rabbit NR1H4 Polyclonal Antibody | anti-NR1H4 antibody
NR1H4 Antibody (N-term)
Western Blot (WB)
(NR1H4 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR1H4 antibody detected the NR1H4 protein (arrow).)
Western Blot (WB)
(NR1H4 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR1H4 antibody detected the NR1H4 protein (arrow).)
Popescu, I.R., et al. FEBS Lett. 584(13):2845-2851(2010)
Gadaleta, R.M., et al. Biochim. Biophys. Acta 1801(7):683-692(2010)
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Erichsen, T.J., et al. J. Hepatol. 52(4):570-578(2010)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a ligand-activated transcription factor, which shares structural features in common with nuclear hormone receptor family, such as a DNA-binding domain that targets the receptor to specific DNA sequences, and a ligand-binding domain, which interacts directly with the ligand and contains a ligand-dependent transcriptional activation domain. This protein functions as a receptor for bile acids, and when bound to bile acids, regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
NR1H4: Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Heterodimer of NR1H4 and RXR. After activation by agonist binding, interacts with a coactivator, NCOA1 or NCOA2. Interacts with CARM1 and SMARD1. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 12q23.1
Cellular Component: nucleoplasm
Molecular Function: ligand-dependent nuclear receptor binding; RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; zinc ion binding; bile acid binding; transcription coactivator activity; peptide binding; retinoid X receptor binding; protein binding; sequence-specific DNA binding; double-stranded DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription corepressor activity
Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; gut development; bile acid metabolic process; response to glucose stimulus; steroid hormone mediated signaling; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; regulation of carbohydrate metabolic process; gene expression; negative regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on NR1H4
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Product Notes
The NR1H4 nr1h4 (Catalog #AAA9205292) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 18-46. The NR1H4 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's NR1H4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the NR1H4 nr1h4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR1H4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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