Rabbit anti-Human NR1H4 Polyclonal Antibody | anti-NR1H4 antibody

NR1H4, NT (NR1H4, BAR, FXR, HRR1, RIP14, Bile acid receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14) (FITC)

Cat. Num. AAA6326515

• Gene Names: NR1H4; BAR; FXR; HRR1; HRR-1; RIP14
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR1H4; Polyclonal Antibody; NT (NR1H4; BAR; FXR; HRR1; RIP14; Bile acid receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14) (FITC); Retinoid X receptor-interacting protein 14; anti-NR1H4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NR1H4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NR1H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human NR1H4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NR1H4 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR1H4 antibody detected the NR1H4 protein (arrow).)

Related Product Information for anti-NR1H4 antibody

NR1H4 is a Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.

Product Categories/Family for anti-NR1H4 antibody

Antibodies; Receptors

References

Meyer Zu Schwabedissen, H.E., et al. Hepatology 52(5):1797-1807(2010). Popescu, I.R., et al. FEBS Lett. 584(13):2845-2851(2010). Gadaleta, R.M., et al. Biochim. Biophys. Acta 1801(7):683-692(2010). Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010). Erichsen, T.J., et al. J. Hepatol. 52(4):570-578(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 332801020
• NCBI GeneID: 9971
• NCBI Accession #: NP_005114.1
• NCBI GenBank Nucleotide #: NM_005123
• UniProt Accession #: Q96RI1; Q8NFP5; Q8NFP6; Q92943; A1L4K5; B7Z412; B7ZM06; F8VYG8
• Molecular Weight: 48,795 Da
• NCBI Official Full Name: Homo sapiens nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 4
• NCBI Official Symbol: NR1H4
• NCBI Official Synonym Symbols: BAR; FXR; HRR1; HRR-1; RIP14
• NCBI Protein Information: bile acid receptor; RXR-interacting protein 14; farnesoid X nuclear receptor; farnesoid X-activated receptor; farnesol receptor HRR-1; retinoid X receptor-interacting protein 14
• UniProt Protein Name: Bile acid receptor
• Protein Family: Bile acid receptor
• UniProt Gene Name: NR1H4
• UniProt Synonym Gene Names: BAR; FXR; HRR1; RIP14; RXR-interacting protein 14
• UniProt Entry Name: NR1H4_HUMAN

NCBI Description

This gene encodes a ligand-activated transcription factor, which shares structural features in common with nuclear hormone receptor family, such as a DNA-binding domain that targets the receptor to specific DNA sequences, and a ligand-binding domain, which interacts directly with the ligand and contains a ligand-dependent transcriptional activation domain. This protein functions as a receptor for bile acids, and when bound to bile acids, regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]