Rabbit CEBPB Polyclonal Antibody | anti-CEBPB antibody

CEBPB Antibody (C-term)

Cat. Num. AAA9204875

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CEBPB; Polyclonal Antibody; CEBPB Antibody (C-term); CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; TCF5; anti-CEBPB antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CEBPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-298 amino acids from the C-terminal region of human CEBPB.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 271-298

• Applicable Applications for anti-CEBPB antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC-P: 1:50~100; FC: 1:10~50
• Function: Important transcription factor regulating theexpression of genes involved in immune andinflammatory responses (PubMed:1741402, PubMed:9374525,PubMed:12048245, PubMed:18647749). Playsalso a significant role in adipogenesis, as wellas in the gluconeogenic pathway, liverregeneration, and hematopoiesis. Theconsensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacityis governed by protein interactions andpost-translational protein modifications.During early embryogenesis, plays essentialand redundant functions with CEBPA. Has apromitotic effect on many cell types such ashepatocytes and adipocytes but has anantiproliferative effect on T-cells byrepressing MYC expression, facilitatingdifferentiation along the T-helper 2 lineage.Binds to regulatory regions of severalacute-phase and cytokines genes and plays arole in the regulation of acute-phase reactionand inflammation. Plays also a role inintracellular bacteria killing (By similarity).During adipogenesis, is rapidly expressedand, after activation by phosphorylation,induces CEBPA and PPARG, which turn on theseries of adipocyte genes that give rise to theadipocyte phenotype. The delayedtransactivation of the CEBPA and PPARGgenes by CEBPB appears necessary to allowmitotic clonal expansion and therebyprogression of terminal differentiation(PubMed:20829347). Essentialfor female reproduction because of a criticalrole in ovarian follicle development (Bysimilarity). Restricts osteoclastogenesis:together with NFE2L1; represses expressionof DSPP during odontoblast differentiation (Bysimilarity).
• Cellular Location: Nucleus. Cytoplasm. Note=Translocates tothe nucleus when phosphorylated at Ser-288.In T-cells when sumoylated drawn topericentric heterochromatin thereby allowingproliferation (By similarity).{ECO:0000250|UniProtKB:P28033,ECO:0000269|PubMed:9374525
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CEBPB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CEBPB Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CEBPB antibody

CEBPB is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. This protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, It can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

Product Categories/Family for anti-CEBPB antibody

Cardiovascular

References

Buck,M., et.al., Mol. Cell 4 (6), 1087-1092 (1999)

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185.2
• NCBI GenBank Nucleotide #: NM_005194.3
• UniProt Accession #: P17676; Q96IH2; Q9H4Z5; A8K671
• Molecular Weight: 36106
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: LAP; LIP; TCF-5
• UniProt Entry Name: CEBPB_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; acute-phase response; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; immune response; negative regulation of neuron apoptosis; inflammatory response; negative regulation of transcription, DNA-dependent