Rabbit anti-Human CEBPB Polyclonal Antibody | anti-CEBPB antibody

CEBPB, CT (CCAAT/Enhancer-binding Protein beta, C/EBP beta, Liver Activator Protein, Nuclear factor NF-IL6, Transcription factor 5, PP9092, TCF5, LAP)

Cat. Num. AAA627347

• Gene Names: CEBPB; LAP; CRP2; TCF5; IL6DBP; NF-IL6; MGC32080; C/EBP-beta
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CEBPB; Polyclonal Antibody; CT (CCAAT/Enhancer-binding Protein beta; C/EBP beta; Liver Activator Protein; Nuclear factor NF-IL6; Transcription factor 5; PP9092; TCF5; LAP); Anti -CEBPB; anti-CEBPB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEBPB.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CEBPB antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human CEBPB (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2) .)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CEBPB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CEBPB Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CEBPB antibody

CEBPB is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. This protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, It can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

Product Categories/Family for anti-CEBPB antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185.2
• NCBI GenBank Nucleotide #: NM_005194.2
• UniProt Accession #: P17676; Q96IH2; Q9H4Z5; A8K671; Q99557; Q9BSC0
• Molecular Weight: 36,106 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: LAP; CRP2; TCF5; IL6DBP; NF-IL6; MGC32080; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta; TCF-5; OTTHUMP00000031262; nuclear factor NF-IL6; transcription factor 5; nuclear factor of interleukin 6; interleukin 6-dependent DNA-binding protein; liver-enriched transcriptional activator protein
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: LAP; TCF5
• UniProt Entry Name: CEBPB_HUMAN

NCBI Description

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; acute-phase response; brown fat cell differentiation; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; inflammatory response