Western Blot (WB)
(Anti-RABEP1 Antibody (Center)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 99 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse RABEP1 Polyclonal Antibody | anti-RABEP1 antibody
RABEP1 Antibody (Center)
Gene Names
RABEP1; RAB5EP; RABPT5
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RABEP1; Polyclonal Antibody; RABEP1 Antibody (Center); Rab GTPase-binding effector protein 1; Rabaptin-4; Rabaptin-5; Rabaptin-5alpha; Renal carcinoma antigen NY-REN-17; RAB5EP; RABPT5; RABPT5A; anti-RABEP1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RABEP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 427-461 amino acids from the Central region of human RABEP1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
829
Applicable Applications for anti-RABEP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-RABEP1 Antibody (Center)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 99 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-RABEP1 Antibody (Center)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 99 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RABEP1 antibody
Rab effector protein acting as linker between gamma- adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A.
References
Stenmark H.,et al.Cell 83:423-432(1995).
Nagelkerken B.,et al.Biochem. J. 346:593-601(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Horiuchi H.,et al.Cell 90:1149-1159(1997).
Nagelkerken B.,et al.Biochem. J. 346:593-601(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Horiuchi H.,et al.Cell 90:1149-1159(1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99290
NCBI Official Full Name
rab GTPase-binding effector protein 1 isoform 2
NCBI Official Synonym Full Names
rabaptin, RAB GTPase binding effector protein 1
NCBI Official Symbol
RABEP1
NCBI Official Synonym Symbols
RAB5EP; RABPT5
NCBI Protein Information
rab GTPase-binding effector protein 1
UniProt Protein Name
Rab GTPase-binding effector protein 1
Protein Family
UniProt Gene Name
RABEP1
UniProt Synonym Gene Names
RAB5EP; RABPT5; RABPT5A
UniProt Entry Name
RABE1_HUMAN
Uniprot Description
RABEP1: Rab effector protein acting as linker between gamma- adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Belongs to the rabaptin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 17p13.2
Cellular Component: recycling endosome; intracellular membrane-bound organelle; endocytic vesicle; early endosome; endosome
Molecular Function: protein binding; protein homodimerization activity; growth factor activity; GTPase activator activity
Biological Process: vesicle-mediated transport; protein transport; apoptosis; endocytosis; positive regulation of GTPase activity
Research Articles on RABEP1
Similar Products
Product Notes
The RABEP1 rabep1 (Catalog #AAA9204735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABEP1 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RABEP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the RABEP1 rabep1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RABEP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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