Western Blot (WB)
(Mouse Dapk3 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Dapk3 antibody detected the Dapk3 protein (arrow).)
Rabbit Dapk3 Polyclonal Antibody | anti-Dapk3 antibody
Mouse Dapk3 Antibody (Center)
Western Blot (WB)
(Mouse Dapk3 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Dapk3 antibody detected the Dapk3 protein (arrow).)
Western Blot (WB)
(Mouse Dapk3 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Dapk3 antibody detected the Dapk3 protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Dapk3 Antibody (Center) (MBS9204223)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Dapk3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Dapk3 Antibody (Center) (MBS9204223)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Dapk3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Ohbayashi, N., et al. Biochem. Biophys. Res. Commun. 372(4):708-712(2008)
Wooldridge, A.A., et al. J. Biol. Chem. 283(17):11850-11859(2008)
Shoval, Y., et al. PLoS Genet. 3(10):1884-1893(2007)
Sato, N., et al. Int. Immunol. 17(12):1543-1552(2005)
NCBI and Uniprot Product Information
Uniprot Description
DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Tumor suppressor; CAMK group; DAPK family
Cellular Component: PML body; cytoskeleton; cytoplasm; chromosome; nucleus; chromosome, pericentric region; lipid raft
Molecular Function: identical protein binding; protein homodimerization activity; Rho GTPase binding; calmodulin-dependent protein kinase activity; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; leucine zipper domain binding; protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding
Biological Process: transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; regulation of myosin II filament assembly or disassembly; regulation of mitotic cell cycle; chromatin modification; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; phosphorylation; positive regulation of cell migration
Research Articles on Dapk3
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Product Notes
The Dapk3 dapk3 (Catalog #AAA9204223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 255-282. The Mouse Dapk3 Antibody (Center) reacts with Mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Dapk3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the Dapk3 dapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dapk3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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