Rabbit anti-Human MASTL Polyclonal Antibody | anti-MASTL antibody

MASTL Antibody

Cat. Num. AAA9203642

• Gene Names: MASTL; GW; GWL; THC2; hGWL; MAST-L; GREATWALL
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MASTL; Polyclonal Antibody; MASTL Antibody; Serine/threonine-protein kinase greatwall; GW; GWL; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L; THC2; anti-MASTL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MASTL antibody is generated from rabbits immunized with human partial MASTL recombinant protein.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 878

• Applicable Applications for anti-MASTL antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Recombinant Protein
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of MASTL Antibody in HepG2, MDA-MB231, 293, Hela cell line lysates (35ug/lane). MASTL (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MASTL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MASTL Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MASTL antibody

MASTL, microtubule associated serine/threonine kinase-like, contains 1 protein kinase domain which belongs to the Ser/Thr protein kinase family. It may be involved in megakaryocyte differentiation. Defects in MASTL are a cause of nonsyndromic autosomal

Product Categories/Family for anti-MASTL antibody

Signal Transduction

References

Gandhi, M.J., et al., Hum. Hered. 55(1):66-70 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 288806587
• NCBI GeneID: 84930
• NCBI Accession #: NP_001165774.1; NP_001165775.1; NP_116233.2
• NCBI GenBank Nucleotide #: NM_001172303.1
• UniProt Accession #: Q96GX5; Q5T8D5; Q5T8D7; Q8NCD6; Q96SJ5
• Molecular Weight: 97319
• NCBI Official Full Name: serine/threonine-protein kinase greatwall isoform 1
• NCBI Official Synonym Full Names: microtubule associated serine/threonine kinase-like
• NCBI Official Symbol: MASTL
• NCBI Official Synonym Symbols: GW; GWL; THC2; hGWL; MAST-L; GREATWALL
• NCBI Protein Information: serine/threonine-protein kinase greatwall
• UniProt Protein Name: Serine/threonine-protein kinase greatwall
• Protein Family: Serine/threonine-protein kinase greatwall
• UniProt Gene Name: MASTL
• UniProt Synonym Gene Names: GW; GWL; THC2; GW; GWL; hGWL; MAST-L
• UniProt Entry Name: GWL_HUMAN

NCBI Description

This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]

Uniprot Description

MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; MAST family

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow

Molecular Function: protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding

Biological Process: mitosis; cell division; regulation of cell cycle; mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; G2/M transition of mitotic cell cycle; response to DNA damage stimulus; protein amino acid phosphorylation

Disease: Thrombocytopenia 2