Western Blot (WB)
(MASTL western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).)
Mouse anti-Human MASTL Monoclonal Antibody | anti-MASTL antibody
MASTL Antibody
IF~~1:10~50
Western Blot (WB)
(MASTL western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).)
Western Blot (WB)
(MASTL western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MASTL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MASTL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
serine/threonine kinase. Mutations at this locus have been
associated with autosomal dominant thrombocytopenia, also known as
thrombocytopenia-2. Alternatively spliced transcript variants have
been described for this locus.
Drachman, J.G., et al. Blood 96(1):118-125(2000)
Savoia, A., et al. Am. J. Hum. Genet. 65(5):1401-1405(1999)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]
Uniprot Description
MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; MAST family
Chromosomal Location of Human Ortholog: 10p12.1
Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow
Molecular Function: protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding
Biological Process: mitosis; cell division; regulation of cell cycle; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; response to DNA damage stimulus; protein amino acid phosphorylation
Disease: Thrombocytopenia 2
Research Articles on MASTL
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Product Notes
The MASTL mastl (Catalog #AAA9200377) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MASTL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MASTL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:100~500 IF~~1:10~50. Researchers should empirically determine the suitability of the MASTL mastl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MASTL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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