Rabbit anti-Human MLYCD Polyclonal Antibody | anti-MLYCD antibody

MLYCD Antibody

Cat. Num. AAA7048672

• Gene Names: MLYCD; MCD
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: MLYCD; Polyclonal Antibody; MLYCD Antibody; Malonyl-CoA decarboxylase; mitochondrial; anti-MLYCD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 493

• Applicable Applications for anti-MLYCD antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 2000-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant human Malonyl-CoA decarboxylase, mitochondrial protein (40-493AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:HEK293 whole cell lysateAll lanes: MLYCD antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 56,51 kDaObserved band size: 56 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7048672 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HepG2 cells using MBS7048672 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-MLYCD antibody

Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Plays a role in the metabolic balance between glucose and lipid oxidation in muscle independent of alterations in insulin signaling. May play a role in controlling the extent of ischemic injury by promoting glucose oxidation.

NCBI and Uniprot Product Information

• NCBI GI #: 110349750
• NCBI GeneID: 23417
• NCBI Accession #: NP_036345.2
• NCBI GenBank Nucleotide #: NM_012213.2
• UniProt Accession #: O95822; Q9UNU5; Q9Y3F2
• Molecular Weight: 50,946 Da
• NCBI Official Full Name: malonyl-CoA decarboxylase, mitochondrial
• NCBI Official Synonym Full Names: malonyl-CoA decarboxylase
• NCBI Official Symbol: MLYCD
• NCBI Official Synonym Symbols: MCD
• NCBI Protein Information: malonyl-CoA decarboxylase, mitochondrial
• UniProt Protein Name: Malonyl-CoA decarboxylase, mitochondrial
• Protein Family: Malonyl-CoA decarboxylase
• UniProt Gene Name: MLYCD
• UniProt Synonym Gene Names: MCD
• UniProt Entry Name: DCMC_HUMAN

NCBI Description

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

MLYCD: Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Defects in MLYCD are the cause of malonyl-CoA decarboxylase deficiency (MLYCD deficiency). MLYCD deficiency is an autosomal recessive disease characterized by abdominal pain, chronic constipation, episodic vomiting, metabolic acidosis and malonic aciduria.

Protein type: Carbohydrate Metabolism - propanoate; EC 4.1.1.9; Lyase; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: malonyl-CoA decarboxylase activity; receptor binding

Biological Process: acetyl-CoA biosynthetic process; acyl-CoA metabolic process; fatty acid biosynthetic process; positive regulation of fatty acid oxidation

Disease: Malonyl-coa Decarboxylase Deficiency