Western Blot (WB)
(NKD2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).)
Rabbit anti-Human NKD2 Polyclonal Antibody | anti-NKD2 antibody
NKD2 Antibody (C-term)
Western Blot (WB)
(NKD2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).)
Western Blot (WB)
(NKD2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).)
Immunohistochemistry (IHC)
(NKD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NKD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NKD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NKD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of NKD2 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of NKD2 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(NKD2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(NKD2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
601365)-binding protein that functions as a negative regulator of
the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf
(see MIM 602272) signaling pathway.
Ding, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(36):13433-13438(2008)
Li, C., et al. Mol. Biol. Cell 18(8):3081-3093(2007)
Adamowicz, M., et al. Genes Chromosomes Cancer 45(9):829-838(2006)
Li, C., et al. Proc. Natl. Acad. Sci. U.S.A. 101(15):5571-5576(2004)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Uniprot Description
NKD2: Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells. Interacts with DVL1, DVL2, DVL3 and PPP2R3A. Interacts with RNF25 and TGFA (via cytoplasmic domain). Expressed in kidney, lung, pancreas and spleen. Belongs to the NKD family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5p15.3
Cellular Component: basolateral plasma membrane; cytoplasmic membrane-bound vesicle; cytoplasmic vesicle; lateral plasma membrane
Molecular Function: protein binding; growth factor binding; ubiquitin protein ligase binding; calcium ion binding
Biological Process: exocytosis; Wnt receptor signaling pathway; Golgi vesicle fusion to target membrane; positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Research Articles on NKD2
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Product Notes
The NKD2 nkd2 (Catalog #AAA9202863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 366-393. The NKD2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NKD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the NKD2 nkd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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