Western Blot (WB)
(GPHN Antibody (Center) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).)
Rabbit GPHN Polyclonal Antibody | anti-GPHN antibody
GPHN Antibody (Center)
Western Blot (WB)
(GPHN Antibody (Center) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).)
Western Blot (WB)
(GPHN Antibody (Center) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).)
Immunohistochemistry (IHC)
(GPHN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GPHN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GPHN Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GPHN Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Fritschy, J.M., et al. Trends Neurosci. 31(5):257-264(2008)
Inada, T., et al. Pharmacogenet. Genomics 18(4):317-323(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Ehrensperger, M.V., et al. Biophys. J. 92(10):3706-3718(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008]
Uniprot Description
gephyrin: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. Homotrimer. Interacts with GABARAP. Inhibited by copper and tungsten. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.10.1.1; EC 2.7.7.75; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 14q23.3
Cellular Component: postsynaptic membrane; cytoskeleton; cytoplasm; plasma membrane; cell junction
Molecular Function: transferase activity; metal ion binding; ATP binding
Biological Process: vitamin metabolic process; molybdenum incorporation into molybdenum-molybdopterin complex; establishment of synaptic specificity at neuromuscular junction; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process
Disease: Molybdenum Cofactor Deficiency, Complementation Group C; Hyperekplexia, Hereditary 1
Research Articles on GPHN
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Product Notes
The GPHN gphn (Catalog #AAA9202611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 437-466. The GPHN Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Chicken, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GPHN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the GPHN gphn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPHN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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