Western Blot (WB)
(PLEKHF1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PLEKHF1 antibody detected the PLEKHF1 protein (arrow).)
Rabbit anti-Human PLEKHF1 Polyclonal Antibody | anti-PLEKHF1 antibody
PLEKHF1 Antibody (N-term)
Gene Names
PLEKHF1; APPD; LAPF; PHAFIN1; ZFYVE15
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PLEKHF1; Polyclonal Antibody; PLEKHF1 Antibody (N-term); Pleckstrin homology domain-containing family F member 1; PH domain-containing family F member 1; Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains; Apoptosis-inducing protein; PH and FYVE domain-containing protein 1; Phafin-1; Zinc finger FYVE domain-containing protein 15; APPD; LAPF; ZFYVE15; anti-PLEKHF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PLEKHF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human PLEKHF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
51-79
Sequence Length
279
Applicable Applications for anti-PLEKHF1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PLEKHF1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PLEKHF1 antibody detected the PLEKHF1 protein (arrow).)
Western Blot (WB)
(PLEKHF1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PLEKHF1 antibody detected the PLEKHF1 protein (arrow).)
Related Product Information for anti-PLEKHF1 antibody
PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Product Categories/Family for anti-PLEKHF1 antibody
References
Chen, W., et al. J. Biol. Chem. 280(49):40985-40995(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31195
NCBI Official Full Name
pleckstrin homology domain-containing family F member 1
NCBI Official Synonym Full Names
pleckstrin homology domain containing, family F (with FYVE domain) member 1
NCBI Official Symbol
PLEKHF1
NCBI Official Synonym Symbols
APPD; LAPF; PHAFIN1; ZFYVE15
NCBI Protein Information
pleckstrin homology domain-containing family F member 1
UniProt Protein Name
Pleckstrin homology domain-containing family F member 1
Protein Family
UniProt Gene Name
PLEKHF1
UniProt Synonym Gene Names
APPD; LAPF; ZFYVE15; PH domain-containing family F member 1; Apoptosis-inducing protein
UniProt Entry Name
PKHF1_HUMAN
Uniprot Description
PKHF1: May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase- independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Chromosomal Location of Human Ortholog: 19q12
Cellular Component: lysosome; perinuclear region of cytoplasm; lysosomal membrane; endosome membrane; nucleus; endosome
Molecular Function: metal ion binding; phosphatidylinositol-5-phosphate binding; phosphatidylinositol 3-phosphate binding
Biological Process: endosome organization and biogenesis; apoptosis; vesicle organization and biogenesis; positive regulation of autophagy
Research Articles on PLEKHF1
Similar Products
Product Notes
The PLEKHF1 plekhf1 (Catalog #AAA9202458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 51-79. The PLEKHF1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLEKHF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PLEKHF1 plekhf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEKHF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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