Western Blot (WB)
(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6013303. This demonstrates that MBS6013303 detected the PLEKHF1 protein (arrow).)
Rabbit anti-Human PLEKHF1 Polyclonal Antibody | anti-PLEKHF1 antibody
PLEKHF1, NT (PLEKHF1, APPD, LAPF, ZFYVE15, Pleckstrin homology domain-containing family F member 1, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, PH and FYVE domain-containing protein 1, Phafin-1, Zinc finger FYVE domain-c
Gene Names
PLEKHF1; APPD; LAPF; PHAFIN1; ZFYVE15
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
PLEKHF1; Polyclonal Antibody; NT (PLEKHF1; APPD; LAPF; ZFYVE15; Pleckstrin homology domain-containing family F member 1; Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains; PH and FYVE domain-containing protein 1; Phafin-1; Zinc finger FYVE domain-c; Zinc finger FYVE domain-containing protein 15; anti-PLEKHF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PLEKHF1.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-PLEKHF1 antibody
Western Blot (WB), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 51-79 in the N-terminal region of human PLEKHF1.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6013303. This demonstrates that MBS6013303 detected the PLEKHF1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6013303. This demonstrates that MBS6013303 detected the PLEKHF1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in human heart lysate (20ug) using MBS6013303 (1:1000). Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 31kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in human heart lysate (20ug) using MBS6013303 (1:1000). Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 31kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PLEKHF1 antibody
PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Product Categories/Family for anti-PLEKHF1 antibody
References
1. Chen, W., et al. J. Biol. Chem. 280(49):40985-40995(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,195 Da
NCBI Official Full Name
pleckstrin homology domain-containing family F member 1
NCBI Official Synonym Full Names
pleckstrin homology and FYVE domain containing 1
NCBI Official Symbol
PLEKHF1
NCBI Official Synonym Symbols
APPD; LAPF; PHAFIN1; ZFYVE15
NCBI Protein Information
pleckstrin homology domain-containing family F member 1
UniProt Protein Name
Pleckstrin homology domain-containing family F member 1
Protein Family
UniProt Gene Name
PLEKHF1
UniProt Synonym Gene Names
APPD; LAPF; ZFYVE15; PH domain-containing family F member 1; Apoptosis-inducing protein
Uniprot Description
PKHF1: May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase- independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Chromosomal Location of Human Ortholog: 19q12
Cellular Component: endosome; endosome membrane; lysosomal membrane; lysosome; nucleus; perinuclear region of cytoplasm
Molecular Function: metal ion binding; phosphatidylinositol 3-phosphate binding; phosphatidylinositol-5-phosphate binding; protein binding
Biological Process: apoptosis; endosome organization and biogenesis; positive regulation of autophagy; vesicle organization and biogenesis
Research Articles on PLEKHF1
Similar Products
Product Notes
The PLEKHF1 plekhf1 (Catalog #AAA6334859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLEKHF1, NT (PLEKHF1, APPD, LAPF, ZFYVE15, Pleckstrin homology domain-containing family F member 1, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, PH and FYVE domain-containing protein 1, Phafin-1, Zinc finger FYVE domain-c reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLEKHF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PLEKHF1 plekhf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEKHF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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