T4 Polynucleotide Kinase DNA Manipulation
T4 Polynucleotide Kinase
Applications
Phosphorylation (Kinase)
Synonyms
T4 Polynucleotide Kinase; T4 Polynucleotide Kinase dna manipulation
Host
E Coli
Form/Format
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 uM ATP pH7.4 at 25 degree C
Concentration
10,000 U/ml (varies by lot)
Applicable Applications for T4 Polynucleotide Kinase dna manipulation
Phosphorylation (Kinase)
Source Note
An E Coli strain that carries the cloned T4 Polynucleotide Kinase gene.
Unit Definition
One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in a total reaction volume of 50 mul in 30 minutes at 37 degree C in 1X T4 PNK Reaction Buffer with 66 uM [gamma-32>P] ATP (5 x 106 cpm/umol) and 0.26 mM 5'-hydroxyl-terminated salmon sperm DNA.
Reaction Conditions
1X T4 PNK Reaction Buffer, Incubate at 37 degree C
Reaction Buffer or Mix
1X T4 PNK Reaction Buffer:
70 mM Tris-HCl, 10 mM MgCl2, 5 mM DTT, pH7.6 at 25 degree C
70 mM Tris-HCl, 10 mM MgCl2, 5 mM DTT, pH7.6 at 25 degree C
Heat Inactivation
65 degree C for 20 min.
Preparation and Storage
Store at -20 degree C.
Related Product Information for T4 Polynucleotide Kinase dna manipulation
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the gamma position of ATP to the 5' -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphoryl polynucleotides, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5'-phosphates to oligonucleotides to allow subsequent ligation and removal of 3'-phosphoryl groups.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5'-phosphates to oligonucleotides to allow subsequent ligation and removal of 3'-phosphoryl groups.
Similar Products
Product Notes
The T4 Polynucleotide Kinase (Catalog #AAA9141652) is a DNA Manipulation produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T4 Polynucleotide Kinase can be used in a range of immunoassay formats including, but not limited to, Phosphorylation (Kinase). Researchers should empirically determine the suitability of the T4 Polynucleotide Kinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T4 Polynucleotide Kinase, DNA Manipulation" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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