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Western Blot (WB) (Western blot-UBE2J1 Polyclonal Antibody)

Rabbit anti-Mouse, Rat UBE2J1 Polyclonal Antibody | anti-UBE2J1 antibody

UBE2J1 Polyclonal Antibody

Gene Names
UBE2J1; UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
UBE2J1; Polyclonal Antibody; UBE2J1 Polyclonal Antibody; CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; UBC6E; Ubc6p; ubiquitin-conjugating enzyme E2 J1; anti-UBE2J1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration
3.13 mg/ml (varies by lot)
Sequence Length
1370
Applicable Applications for anti-UBE2J1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2).
Immunogen Sequence
METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPE
Positive Samples
Mouse Heart, Mouse Kidney, Rat Lung
Cellular Location
Endoplasmic Reticulum Membrane, Single-Pass Type IV Membrane Protein
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot-UBE2J1 Polyclonal Antibody)

Western Blot (WB) (Western blot-UBE2J1 Polyclonal Antibody)
Related Product Information for anti-UBE2J1 antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated: 35kDa
Observed: 35kDa
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast), mRNA
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 J1
NCBI Official Symbol
UBE2J1
NCBI Official Synonym Symbols
UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243
NCBI Protein Information
ubiquitin-conjugating enzyme E2 J1
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 J1
UniProt Gene Name
UBE2J1
UniProt Synonym Gene Names
NCUBE1; NCUBE-1; HsUBC6e
UniProt Entry Name
UB2J1_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). Ref.2 Ref.15

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway: Protein modification; protein ubiquitination.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.2.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution: The sequence AAD34071.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF36125.1 differs from that shown. Reason: Frameshift at position 119.

Research Articles on UBE2J1

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Product Notes

The UBE2J1 ube2j1 (Catalog #AAA9140549) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2J1 Polyclonal Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2J1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the UBE2J1 ube2j1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2J1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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