Typical Testing Data/Standard Curve (for reference only)
Pig high-mobility group box 1 ELISA Kit | HMGB1 elisa kit
Pig High mobility group protein B1, HMGB1 ELISA Kit
Reactivity
Pig
Synonyms
high-mobility group box 1; Pig High mobility group protein B1; HMGB1 ELISA Kit; Pig High mobility group protein B1 (HMGB1) ELISA kit; DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot; HMGB1 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of pig HMGB1. No significant cross-reactivity or interference between pig HMGB1 and analogues was observed.
Sequence Length
215
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
< 0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HMGB1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,917 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Symbol
HMGB1
NCBI Protein Information
high mobility group protein B1; HMG-1; non-histone protein HMG1; high-mobility group box 1; high mobility group protein 1
UniProt Protein Name
High mobility group protein B1
Protein Family
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_PIG
Uniprot Description
Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells
Research Articles on HMGB1
Similar Products
Product Notes
The Pig HMGB1 hmgb1 (Catalog #AAA913688) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA913688 ELISA Kit recognizes Pig HMGB1. It is sometimes possible for the material contained within the vial of "high-mobility group box 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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