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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MTIF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Rabbit MTIF3 Polyclonal Antibody | anti-MTIF3 antibody

MTIF3 Polyclonal Antibody

Gene Names
MTIF3; IF3mt
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
MTIF3; Polyclonal Antibody; MTIF3 Polyclonal Antibody; IF3mt; anti-MTIF3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
278
Applicable Applications for anti-MTIF3 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 2000
Immunogen
Recombinant protein of human MTIF3
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MTIF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MTIF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-MTIF3 antibody
This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.
Product Categories/Family for anti-MTIF3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
32kDa
NCBI Official Full Name
Translation initiation factor IF-3, mitochondrial
NCBI Official Synonym Full Names
mitochondrial translational initiation factor 3
NCBI Official Symbol
MTIF3
NCBI Official Synonym Symbols
IF3mt
NCBI Protein Information
translation initiation factor IF-3, mitochondrial
UniProt Protein Name
Translation initiation factor IF-3, mitochondrial
UniProt Gene Name
MTIF3
UniProt Synonym Gene Names
IF-3(Mt); IF-3Mt; IF3(mt); IF3mt

NCBI Description

This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Oct 2009]

Uniprot Description

MTIF3: IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins. Belongs to the IF-3 family.

Protein type: Mitochondrial; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: mitochondrion

Molecular Function: protein binding; ribosomal small subunit binding; translation factor activity, nucleic acid binding

Biological Process: ribosome disassembly

Research Articles on MTIF3

Similar Products

Product Notes

The MTIF3 mtif3 (Catalog #AAA9132508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTIF3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MTIF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 2000. Researchers should empirically determine the suitability of the MTIF3 mtif3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTIF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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