Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ID2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit anti-Human ID2 Polyclonal Antibody | anti-ID2 antibody
ID2 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ID2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ID2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
Uniprot Description
ID2: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2. Highly expressed in early fetal tissues, including those of the central nervous system.
Protein type: DNA-binding; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 12 A1.3|12 8.57 cM
Cellular Component: chromatin; cytoplasm; cytosol; nucleus; protein complex
Molecular Function: protein binding
Biological Process: adult locomotory behavior; cell development; cell maturation; circadian regulation of gene expression; circadian rhythm; embryonic digestive tract morphogenesis; entrainment of circadian clock; entrainment of circadian clock by photoperiod; enucleate erythrocyte differentiation; heart development; leukocyte differentiation; locomotor rhythm; lymph node development; mammary gland epithelial cell proliferation; metanephros development; natural killer cell differentiation; negative regulation of B cell differentiation; negative regulation of DNA binding; negative regulation of neuron differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of osteoblast differentiation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron fate commitment; neuron morphogenesis during differentiation; olfactory bulb development; oligodendrocyte development; Peyer's patch development; positive regulation of astrocyte differentiation; positive regulation of blood pressure; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of erythrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of macrophage differentiation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; regulation of circadian rhythm; regulation of lipid metabolic process; thigmotaxis
Research Articles on ID2
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Product Notes
The ID2 id2 (Catalog #AAA9132018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ID2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ID2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 2000. Researchers should empirically determine the suitability of the ID2 id2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ID2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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