Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-P70S6K1-T421/S424 antibody at 1:2000 dilution. 293T cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human Phospho-P70S6K1-T421/S424 Antibody | anti-P70S6K1-T421/S424 antibody
Phospho-P70S6K1-T421/S424 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-P70S6K1-T421/S424 antibody at 1:2000 dilution. 293T cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-P70S6K1-T421/S424 antibody at 1:2000 dilution. 293T cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
Molecular Weight: 525
NCBI Description
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
Uniprot Description
p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Translation; Protein kinase, AGC; Kinase, protein; AGC group; RSK family; p70 subfamily
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: cell junction; cell surface; cytoplasm; cytosol; mitochondrial outer membrane; mitochondrion; neuron projection; nucleoplasm; nucleus; perinuclear region of cytoplasm; synapse
Molecular Function: ATP binding; identical protein binding; PDZ domain binding; peptide binding; protein binding; protein kinase activity; protein phosphatase 2A binding; protein serine/threonine/tyrosine kinase activity; ribosomal protein S6 kinase activity
Biological Process: aging; apoptosis; behavioral fear response; cell migration; cellular response to insulin stimulus; G1/S transition of mitotic cell cycle; germ cell development; insulin receptor signaling pathway; long-term memory; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitotic cell cycle; positive regulation of skeletal muscle growth; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of translation; positive regulation of translational initiation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of glucose import; response to drug; response to electrical stimulus involved in regulation of muscle adaptation; response to ethanol; response to glucagon stimulus; response to glucocorticoid stimulus; response to glucose stimulus; response to heat; response to leucine; response to lipopolysaccharide; response to mechanical stimulus; response to nutrient; response to testosterone stimulus; response to toxin; response to wounding; signal transduction; skeletal muscle atrophy; skeletal muscle contraction; TOR signaling pathway
Research Articles on P70S6K1-T421/S424
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Product Notes
The P70S6K1-T421/S424 rps6kb1 (Catalog #AAA9129004) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-P70S6K1-T421/S424 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-P70S6K1-T421/S424 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the P70S6K1-T421/S424 rps6kb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-P70S6K1-T421/S424, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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