Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GALNT2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Rabbit GALNT2 Polyclonal Antibody | anti-GALNT2 antibody
GALNT2 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GALNT2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GALNT2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using GALNT2 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using GALNT2 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using GALNT2 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using GALNT2 Antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using GALNT2 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using GALNT2 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
GALNT2: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b.
Protein type: Membrane protein, integral; EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Transferase
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; perinuclear region of cytoplasm; integral to Golgi membrane
Molecular Function: manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity; carbohydrate binding
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; protein amino acid O-linked glycosylation via threonine; immunoglobulin biosynthetic process; post-translational protein modification; protein amino acid O-linked glycosylation via serine
Research Articles on GALNT2
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Product Notes
The GALNT2 galnt2 (Catalog #AAA9127671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GALNT2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the GALNT2 galnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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