Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DAP Kinase 1 (DAP Kinase 1 (DAPK1))Antibody (MBS9127324) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 30s.)
Rabbit DAPK1 Polyclonal Antibody | anti-DAPK1 antibody
DAPK1 Polyclonal Antibody
IHC: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DAP Kinase 1 (DAP Kinase 1 (DAPK1))Antibody (MBS9127324) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DAP Kinase 1 (DAP Kinase 1 (DAPK1))Antibody (MBS9127324) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 30s.)
NCBI and Uniprot Product Information
Calculated MW: 36 kDa/ 152 kDa/ 160 kDa/ 161 kDa
NCBI Description
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Uniprot Description
DAPK1: a CAMK protein kinase of the Death-associated protein kinase (DAPK) family. Mediates autophagy and is a positive mediator of ER stress-induced and gamma-interferon induced programmed cell death. Catalytic activity is increased by ER stress. Inhibited by autophosphorylation of S308. DAPK -/- cells are protected from ER stress-induced cell death, and both apoptosis and autophagy are decreased. Colocalizes with the actin filament system. It is a tumor suppressor candidate, activating p53-dependent apoptosis. Expression is reduced in some cancers, and the promoter is frequently hypermethylated in invasive cancers. Increased expression and mislocalization is seen in epilepsy, where it may regulate neuronal death. A structurally unique serine-threonine kinase that carries eight ankyrin repeats and two putative P-loop consensus sites. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, CAMK; Autophagy; Kinase, protein; Apoptosis; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family
Chromosomal Location of Human Ortholog: 9q21.33
Cellular Component: cytoplasm; plasma membrane; actin cytoskeleton
Molecular Function: calmodulin binding; identical protein binding; protein serine/threonine kinase activity; protein binding; calmodulin-dependent protein kinase activity; syntaxin-1 binding; ATP binding; protein kinase activity
Biological Process: regulation of apoptosis; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; negative regulation of translation; protein amino acid autophosphorylation; positive regulation of caspase activity; regulation of autophagy; protein amino acid phosphorylation
Research Articles on DAPK1
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Product Notes
The DAPK1 dapk1 (Catalog #AAA9127324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DAPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the DAPK1 dapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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