Rabbit anti-Human, Mouse TLK2 Polyclonal Antibody | anti-TLK2 antibody
TLK2 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver injury using TLK2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
TLK2: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; TLK family
Chromosomal Location of Human Ortholog: 17q23
Cellular Component: perinuclear region of cytoplasm; intermediate filament; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: negative regulation of autophagy; peptidyl-serine phosphorylation; regulation of chromatin assembly or disassembly; chromatin modification; cell cycle; response to DNA damage stimulus; protein amino acid phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; chromosome segregation