Mouse CD163 molecule ELISA Kit | CD163 elisa kit

Mouse Scavenger receptor cysteine-rich type 1 protein M130, CD163 ELISA Kit

Cat. Num. AAA903944

• Gene Names: Cd163; CD163v2; CD163v3
• Reactivity: Mouse
• Synonyms: CD163 molecule; Mouse Scavenger receptor cysteine-rich type 1 protein M130; CD163 ELISA Kit; Mouse Scavenger receptor cysteine-rich type 1 protein M130 (CD163) ELISA kit; M130; MM130; CD163 antigen; macrophage-associated antigen; CD163 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse CD163. No significant cross-reactivity or interference between mouse CD163 and analogues was observed.
• Sequence Length: 1121

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml
• Sensitivity: 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD163 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD163 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD163 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD163 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD163 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 281371421
• NCBI GeneID: 93671
• NCBI Accession #: NP_444324.2
• NCBI GenBank Nucleotide #: NM_053094.2
• UniProt Accession #: Q2VLH6; Q2VLH5; Q99MX8; A6H691
• Molecular Weight: 120,921 Da
• NCBI Official Full Name: scavenger receptor cysteine-rich type 1 protein M130 isoform 2
• NCBI Official Synonym Full Names: CD163 antigen
• NCBI Official Symbol: Cd163
• NCBI Official Synonym Symbols: CD163v2; CD163v3
• NCBI Protein Information: scavenger receptor cysteine-rich type 1 protein M130
• UniProt Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Protein Family: Scavenger receptor cysteine-rich type 1 protein
• UniProt Gene Name: Cd163
• UniProt Synonym Gene Names: M130; sCD163
• UniProt Entry Name: C163A_MOUSE

Uniprot Description

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response; inflammatory response