anti-Human TTF1 Antibody | anti-TTF1 antibody

TTF1 Antibody

Cat. Num. AAA859842

• Gene Names: TTF1; TTF-1; TTF-I
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TTF1; TTF1 Antibody; TTF-1; TTF-I; RNA polymerase I termination factor; anti-TTF1 antibody

• Reactivity: Human
• Specificity: TTF1 antibody detects endogenous levels of total TTF1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 390

• Applicable Applications for anti-TTF1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human TTF1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(Peptide - +Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TTF1 antibody.)

References

Evers R., Proc. Natl. Acad. Sci. U.S.A. 92:5827-5831(1995). Humphray S.J., Nature 429:369-374(2004). Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 328927005
• NCBI GeneID: 7270
• NCBI Accession #: NP_001192225.1
• NCBI GenBank Nucleotide #: NM_001205296.1
• UniProt Accession #: Q15361; Q4VXF3; Q58EY2; Q6P5T5; A1L160
• Molecular Weight: 103,051 Da
• NCBI Official Full Name: transcription termination factor 1 isoform 2
• NCBI Official Synonym Full Names: transcription termination factor 1
• NCBI Official Symbol: TTF1
• NCBI Official Synonym Symbols: TTF-1; TTF-I
• NCBI Protein Information: transcription termination factor 1
• UniProt Protein Name: Transcription termination factor 1
• Protein Family: Transcription termination factor
• UniProt Gene Name: TTF1
• UniProt Synonym Gene Names: TTF-1; TTF-I

NCBI Description

This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011]

Uniprot Description

Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.