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Testing Data
(Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 15ug/ml (clone: DM12) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Rabbit anti-Human CD22 Monoclonal Antibody | anti-CD22 antibody
Anti-CD22 antibody (DM12), Rabbit mAb
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
CD22; Monoclonal Antibody; Anti-CD22 antibody (DM12); Rabbit mAb; SIGLEC-2; SIGLEC2; anti-CD22 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM12
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Liquid
Preservative: 0.1%Procline 300
Constituents: 50%Glycerol; PBS, pH7.4; 0.1%BSA
Preservative: 0.1%Procline 300
Constituents: 50%Glycerol; PBS, pH7.4; 0.1%BSA
Applicable Applications for anti-CD22 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF)
Application Notes
FC/FACS: 1:100
Immunogen
Recombinant human CD22 (Asp20-Arg687) produced by using human HEK293 cells
Preparation and Storage
Store at -20 degree C for 15 months (Avoid repeated freezing and thawing)
Shipping: Ice Pack
Shipping: Ice Pack
Testing Data
(Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 15ug/ml (clone: DM12) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Testing Data
(Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 15ug/ml (clone: DM12) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Flow Cytometry (FC/FACS)
(Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM12) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Flow Cytometry (FC/FACS)
(Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM12) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.)
Testing Data
(Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.)
Related Product Information for anti-CD22 antibody
CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC1.
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Product Notes
The CD22 (Catalog #AAA8579074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD22 antibody (DM12), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD22 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF). FC/FACS: 1:100. Researchers should empirically determine the suitability of the CD22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD22, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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