Mouse anti-Human SCAP Monoclonal Antibody | anti-SCAP antibody

SCAP (N-Term) Mouse mAb

Cat. Num. AAA8558623

• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A/G
• Synonyms: SCAP; Monoclonal Antibody; SCAP (N-Term) Mouse mAb; anti-SCAP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Purity/Purification: Protein A/G
• Form/Format: PBS with 0.09% (W/V) Sodium Azide, pH 7.3.
• Sequence Length: 1279

• Applicable Applications for anti-SCAP antibody: Western Blot (WB)
• Application Notes: WB: 1:4000
• Immunogen: This SCAP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the N-terminal region of human SCAP.
• Cellular Localization: Cytoplasmic Vesicle, Endoplasmic Reticulum, Golgi Apparatus, Membrane
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Related Product Information for anti-SCAP antibody

Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 66932902
• NCBI GeneID: 22937
• NCBI Accession #: NP_036367.2
• NCBI GenBank Nucleotide #: NP_036367.2
• UniProt Accession #: Q12770
• NCBI Official Full Name: sterol regulatory element-binding protein cleavage-activating protein isoform 1
• NCBI Official Synonym Full Names: SREBF chaperone
• NCBI Official Symbol: SCAP
• NCBI Protein Information: sterol regulatory element-binding protein cleavage-activating protein
• UniProt Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Protein Family: Sterol regulatory element-binding protein cleavage-activating protein
• UniProt Gene Name: SCAP
• UniProt Synonym Gene Names: KIAA0199; SCAP; SREBP cleavage-activating protein
• UniProt Entry Name: SCAP_HUMAN

NCBI Description

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; unfolded protein binding; cholesterol binding; protein complex binding

Biological Process: cholesterol metabolic process; regulation of fatty acid biosynthetic process; response to hypoxia; positive regulation of low-density lipoprotein receptor biosynthetic process; SREBP-mediated signaling pathway; negative regulation of cholesterol biosynthetic process; response to insulin stimulus; aging