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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Annexin A6 antibody.)

anti-Human Annexin A6 Antibody | anti-ANX6 antibody

Annexin A6 Antibody

Gene Names
ANXA6; p68; p70; ANX6; CBP68; CPB-II
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Annexin A6; Annexin A6 Antibody; 67 kDa calelectrin; Annexin VI; CPB-II; Calphobindin-II; Chromobindin 20; Lipocortin VI; P68; P70; Protein III; annexin A6; anti-ANX6 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Annexin A6 antibody detects endogenous levels of total Annexin A6 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
673
Applicable Applications for anti-ANX6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from human Annexin A6.
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Annexin A6 antibody.)

Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Annexin A6 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using Annexin A6 antibody.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells, using Annexin A6 antibody.)

Immunofluorescence (IF)

(Forskolin +- Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using Annexin A6 antibody (#30006).)

Immunofluorescence (IF) (Forskolin +- Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using Annexin A6 antibody (#30006).)
References
Ning Chang, Am J Physiol Cell Physiol, Apr 2007; 292: C1417-C1430. E Smythe, J. Cell Biol., Feb 1994; 124: 301. J. M. Naciff, Am J Physiol Cell Physiol, Dec 1996; 271: C2004-C2015. Iñaki de Diego, J. Biol. Chem., Aug 2002; 277: 32187-32194.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
309
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,423 Da
NCBI Official Full Name
annexin A6 isoform 1
NCBI Official Synonym Full Names
annexin A6
NCBI Official Symbol
ANXA6
NCBI Official Synonym Symbols
p68; p70; ANX6; CBP68; CPB-II
NCBI Protein Information
annexin A6
UniProt Protein Name
Annexin A6
Protein Family
UniProt Gene Name
ANXA6
UniProt Synonym Gene Names
ANX6; CPB-II

NCBI Description

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010]

Uniprot Description

May associate with CD21. May regulate the release of Ca2+ from intracellular stores.

Research Articles on ANX6

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Product Notes

The ANX6 anxa6 (Catalog #AAA855790) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Annexin A6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the ANX6 anxa6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A6, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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