Rabbit CIB1 Polyclonal Antibody | anti-CIB1 antibody

CIB1 Rabbit pAb

Cat. Num. AAA8545385

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: CIB1; Polyclonal Antibody; CIB1 Rabbit pAb; CIB; CIBP; KIP1; PRKDCIP; SIP2-28; anti-CIB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
• Sequence Length: 191

• Applicable Applications for anti-CIB1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CIB1 (NP_006375.2).
• Cellular Localization: Cell Membrane, Cell Projection, Cytoplasm, Cytoskeleton, Golgi Apparatus, Membrane, Nucleus
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-CIB1 antibody

Calcium-binding protein that plays a role in the regulation of numerous cellular processes, such as cell differentiation, cell division, cell proliferation, cell migration, thrombosis, angiogenesis, cardiac hypertrophy and apoptosis. Involved in bone marrow megakaryocyte differentiation by negatively regulating thrombopoietin-mediated signaling pathway. Participates in the endomitotic cell cycle of megakaryocyte, a form of mitosis in which both karyokinesis and cytokinesis are interrupted. Plays a role in integrin signaling by negatively regulating alpha-IIb/beta3 activation in thrombin-stimulated megakaryocytes preventing platelet aggregation. Up-regulates PTK2/FAK1 activity, and is also needed for the recruitment of PTK2/FAK1 to focal adhesions; it thus appears to play an important role in focal adhesion formation. Positively regulates cell migration on fibronectin in a CDC42-dependent manner, the effect being negatively regulated by PAK1. Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. Down-regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Involved in sphingosine kinase SPHK1 translocation to the plasma membrane in a N-myristoylation-dependent manner preventing TNF-alpha-induced apoptosis. Regulates serine/threonine-protein kinase PLK3 activity for proper completion of cell division progression. Plays a role in microtubule (MT) dynamics during neuronal development; disrupts the MT depolymerization activity of STMN2 attenuating NGF-induced neurite outgrowth and the MT reorganization at the edge of lamellipodia. Promotes cardiomyocyte hypertrophy via activation of the calcineurin/NFAT signaling pathway. Stimulates calcineurin PPP3R1 activity by mediating its anchoring to the sarcolemma. In ischemia-induced (pathological or adaptive) angiogenesis, stimulates endothelial cell proliferation, migration and microvessel formation by activating the PAK1 and ERK1/ERK2 signaling pathway. Promotes also cancer cell survival and proliferation. May regulate cell cycle and differentiation of spermatogenic germ cells, and/or differentiation of supporting Sertoli cells.Isoform 2: Plays a regulatory role in angiogenesis and tumor growth by mediating PKD/PRKD2-induced vascular endothelial growth factor A (VEGFA) secretion.

NCBI and Uniprot Product Information

• NCBI GI #: 134047806
• UniProt Accession #: Q99828
• Molecular Weight: 20kDa
• NCBI Official Full Name: Calcium and integrin-binding protein 1
• UniProt Protein Name: Calcium and integrin-binding protein 1
• Protein Family: Calcium and integrin-binding protein
• UniProt Gene Name: CIB1
• UniProt Synonym Gene Names: CIB; KIP; PRKDCIP; CIB; CIBP; KIP; SIP2-28
• UniProt Entry Name: CIB1_HUMAN

Uniprot Description

CIB1: May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.

Protein type: Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 15q25.3-q26

Cellular Component: Golgi apparatus; filopodium tip; centrosome; neuron projection; endoplasmic reticulum; nucleoplasm; growth cone; membrane; cell soma; perinuclear region of cytoplasm; lamellipodium; cytoplasm; apical plasma membrane; plasma membrane; nucleus; sarcolemma; vesicle

Molecular Function: protein binding; Ras GTPase binding; protein anchor; calcium ion binding

Biological Process: negative regulation of protein kinase B signaling cascade; apoptosis; negative regulation of microtubule depolymerization; positive regulation of cell adhesion mediated by integrin; positive regulation of metalloenzyme activity; endomitotic cell cycle; positive regulation of cell growth; negative regulation of megakaryocyte differentiation; cytoplasmic microtubule organization and biogenesis; activation of NF-kappaB transcription factor; regulation of cell proliferation; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; cell division; double-strand break repair; positive regulation of cell proliferation; angiogenesis; positive regulation of protein amino acid phosphorylation; platelet formation; positive regulation of cell-matrix adhesion; cell adhesion; response to DNA damage stimulus; regulation of cell division; spermatid development; positive regulation of cell migration; negative regulation of apoptosis