Western Blot (WB)
(Western blot analysis of Jurkat cell lysate using F4-80 antibody.)
Rabbit anti-Human F4-80 Polyclonal Antibody | anti-F4-80 antibody
F4-80 Polyclonal Antibody
Gene Names
Adgre1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
F4-80; Polyclonal Antibody; F4-80 Polyclonal Antibody; Emr1; DD7A5-7; EGF-TM7; F4/80; Gpf480; Ly71; TM7LN3; anti-F4-80 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
931
Applicable Applications for anti-F4-80 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
A synthetic peptide of human F4-80
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Jurkat cell lysate using F4-80 antibody.)
Western Blot (WB)
(Western blot analysis of Jurkat cell lysate using F4-80 antibody.)
Related Product Information for anti-F4-80 antibody
The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1-3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1-3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1, 3, 4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5, 6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages (1).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
102kDa
NCBI Official Full Name
adhesion G protein-coupled receptor E1 isoform 1
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor E1
NCBI Official Symbol
Adgre1
NCBI Official Synonym Symbols
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
NCBI Protein Information
adhesion G protein-coupled receptor E1
UniProt Protein Name
Adhesion G protein-coupled receptor E1
UniProt Gene Name
Adgre1
UniProt Synonym Gene Names
Emr1; Gpf480
Uniprot Description
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Research Articles on F4-80
Similar Products
Product Notes
The F4-80 adgre1 (Catalog #AAA8527364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The F4-80 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's F4-80 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the F4-80 adgre1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F4-80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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