Rabbit anti-Human RNF217 Polyclonal Antibody | anti-RNF217 antibody

RNF217, NT (RNF217, C6orf172, IBRDC1, Probable E3 ubiquitin-protein ligase RNF217, IBR domain-containing protein 1, RING finger protein 217) (AP)

Cat. Num. AAA6342830

• Gene Names: RNF217; IBRDC1; C6orf172; dJ84N20.1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF217; Polyclonal Antibody; NT (RNF217; C6orf172; IBRDC1; Probable E3 ubiquitin-protein ligase RNF217; IBR domain-containing protein 1; RING finger protein 217) (AP); RING finger protein 217; anti-RNF217 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RNF217 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RNF217 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human RNF217.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RNF217 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RNF217 antibody detected the RNF217 protein (arrow).)

Related Product Information for anti-RNF217 antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity).

Product Categories/Family for anti-RNF217 antibody

Antibodies; Ubiquitin Ligases

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008). Mungall, A.J., et al. Nature 425(6960):805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 22749145
• NCBI GeneID: 154214
• NCBI Accession #: NP_689766.1
• NCBI GenBank Nucleotide #: NM_152553.3
• UniProt Accession #: Q8TC41; Q5TCA4; Q9BX48; H7C5V4
• Molecular Weight: 59372
• NCBI Official Full Name: probable E3 ubiquitin-protein ligase RNF217 isoform b
• NCBI Official Synonym Full Names: ring finger protein 217
• NCBI Official Symbol: RNF217
• NCBI Official Synonym Symbols: IBRDC1; C6orf172; dJ84N20.1
• NCBI Protein Information: probable E3 ubiquitin-protein ligase RNF217
• UniProt Protein Name: Probable E3 ubiquitin-protein ligase RNF217
• Protein Family: Probable E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF217
• UniProt Synonym Gene Names: C6orf172; IBRDC1

Uniprot Description

RNF217: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Belongs to the RBR family. RNF217 subfamily.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: cytoplasm; cytosol; integral component of membrane; ubiquitin ligase complex

Molecular Function: metal ion binding; ubiquitin conjugating enzyme binding; ubiquitin-protein transferase activity

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination involved in ubiquitin-dependent protein catabolic process