anti-Human Caspase 1 Antibody | anti-CASP-1 antibody

Caspase 1 (Cleaved-Asp210) Antibody

Cat. Num. AAA851976

• Gene Names: CASP1; ICE; P45; IL1BC
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Caspase 1; Caspase 1 (Cleaved-Asp210) Antibody; ICE; IL-1 beta converting enzyme; IL-1BC; IL1BCE; caspase-1; interleukin-1 beta convertase; interleukin-1 beta converting enzyme; p45; anti-CASP-1 antibody

• Reactivity: Human
• Specificity: Caspase 1 (Cleaved-Asp210) antibody detects endogenous levels of fragment of activated Caspase 1 resulting from cleavage adjacent to Asp210.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 383

• Applicable Applications for anti-CASP-1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 1(MTTELEAFC).
• All Sites: Human: Asp210
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Cl-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 1 (Cleaved-Asp210) antibody.)

References

Victor Elharrrar, FASEB J, Mar 2006; 20: A228. R Matsumura, Ann Rheum Dis, May 2001; 60: 473-482. Adriana López Ponte Stem Cells, Mar 2007; 10.1634/stemcells.2007-0054. A I Catrina, Ann Rheum Dis, Oct 2002; 61: 934-936.

NCBI and Uniprot Product Information

• NCBI GI #: 4502573
• NCBI GeneID: 834
• NCBI Accession #: NP_001214.1
• NCBI GenBank Nucleotide #: NM_001223.4
• UniProt Accession #: P29466; Q53EY6; Q6DMQ1; Q6GSS3; Q6PI75; Q9UCN3; B5MDZ1
• Molecular Weight: 10,417 Da
• NCBI Official Full Name: caspase-1 isoform beta
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: CASP1
• NCBI Official Synonym Symbols: ICE; P45; IL1BC
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).