Western Blot (WB)
(Figure 1. Western blot analysis using KLHL11 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.)
Mouse anti-Human KLHL11 Antibody | anti-KLHL11 antibody
KLHL11 Antibody
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
KLHL11; KLHL11 Antibody; FLJ10572; anti-KLHL11 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
1B9A8
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
708
Applicable Applications for anti-KLHL11 antibody
Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human KLHL11 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using KLHL11 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.)
Western Blot (WB)
(Figure 1. Western blot analysis using KLHL11 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.)
Immunofluorescence (IF)
(Figure 2. Immunofluorescence analysis of 3T3-L1 cells using KLHL11 mouse mAb (green). Blue. DRAQ5 fluorescent DNA dye. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Figure 2. Immunofluorescence analysis of 3T3-L1 cells using KLHL11 mouse mAb (green). Blue. DRAQ5 fluorescent DNA dye. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometric analysis of K562 cells using KLHL11 mouse mAb (green) and negative control (purple).)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometric analysis of K562 cells using KLHL11 mouse mAb (green) and negative control (purple).)
Related Product Information for anti-KLHL11 antibody
Kelch-like protein 11, Subcellular location Secreted.
Product Categories/Family for anti-KLHL11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80kDa
NCBI Official Full Name
kelch-like protein 11
NCBI Official Synonym Full Names
kelch like family member 11
NCBI Official Symbol
KLHL11
NCBI Protein Information
kelch-like protein 11
UniProt Protein Name
Kelch-like protein 11
Protein Family
UniProt Gene Name
KLHL11
Uniprot Description
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.
Research Articles on KLHL11
1. analysis of crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3
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Product Notes
The KLHL11 klhl11 (Catalog #AAA850973) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KLHL11 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLHL11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IF: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the KLHL11 klhl11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL11, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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