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Western Blot (WB) (Figure 1. Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.)

Mouse anti-Human TUBB3 Antibody | anti-TUBB3 antibody

TUBB3 Antibody

Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
TUBB3; TUBB3 Antibody; Tubulin; beta 3; MC1R; TUBB4; anti-TUBB3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
2E9
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
378
Applicable Applications for anti-TUBB3 antibody
Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human TUBB3 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.)

Western Blot (WB) (Figure 1. Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.)

Immunofluorescence (IF)

(Figure 2. Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue. DRAQ5 fluorescent DNA dye. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Immunofluorescence (IF) (Figure 2. Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue. DRAQ5 fluorescent DNA dye. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Flow Cytometry (FC/FACS)

(Figure 3. Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and negative control (purple).)

Flow Cytometry (FC/FACS) (Figure 3. Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and negative control (purple).)
Related Product Information for anti-TUBB3 antibody
Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, alpha-and beta-tubulin, are usually products of separate genes. The beta-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III beta-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III beta-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50kDa
NCBI Official Full Name
tubulin beta-3 chain isoform 2
NCBI Official Synonym Full Names
tubulin beta 3 class III
NCBI Official Symbol
TUBB3
NCBI Official Synonym Symbols
CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
NCBI Protein Information
tubulin beta-3 chain
UniProt Protein Name
Tubulin beta-3 chain
Protein Family
UniProt Gene Name
TUBB3
UniProt Synonym Gene Names
TUBB4

NCBI Description

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Research Articles on TUBB3

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Product Notes

The TUBB3 tubb3 (Catalog #AAA850475) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TUBB3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TUBB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IF: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the TUBB3 tubb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBB3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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