IFN-gamma elisa kit

Human IFN-gamma ELISA KIT

Cat. Num. AAA8506073

• Gene Names: IFNG; IFG; IFI
• Synonyms: IFN-gamma; Human IFN-gamma ELISA KIT; IFN-gamma elisa kit

• Specificity: This assay recognizes both natural and recombinant human IFN-gamma. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
• Sequence Length: 166

• Samples: Cell culture supernates, serum, and plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: 7pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFN-gamma elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-gamma is added to the wells and binds to the combination of capture antibody- IFN-gamma in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IFN-gamma present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-gamma standard dilutions and IFN-gamma sample concentration determined.!!Background/Introduction: Interferon gamma (IFN-gamma) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA (1). Produced by Tlymphocytes and natural killer (NK) cells, IFN-gamma gammais now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-gammagamma is reported to be active only on human and non-human primate cells (5). The biochemistry and biological activities of the interferons have been extensively reviewed (2-9). Human IFN-gammagammais a 143 amino acid residue, 20 or 25 kDa glycoprotein that demonstrates little sequence homology to IFN-alphagammaor -betagamma(10-13). Naturally occurring IFN-gamma is found as either of two molecular weight species, differing in degree of glycosylation. Human IFN-gamma apparently exists as a head-to-tail dimer in solution with the C-terminus of one monomer aligned with the N-terminus of the other monomer (14,15) A receptor for IFN-gamma has been identified and its gene localized to chromosome 6 (16,17) Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-gamma(18-21). Functionally, IFN-gamma produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-gamma has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes (9, 22-25) he antiprotozoal activity of IFN-gamma against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-gamma (26).The immunomodulatory effects of IFN-gammagamma are extensive and diverse. In monocyte/macrophages, the activities of IFN-gamma gammainclude: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-gammagammahas been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• UniProt Accession #: P01579; Q53ZV4; B5BU88
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Uniprot Description

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.