Mouse HSP70 Antibody | anti-HSP70 antibody

HSP70 Antibody

Cat. Num. AAA8505073

• Gene Names: HSPA1B; HSP70-2; HSP70.2; HSP70-1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: HSP70; HSP70 Antibody; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HSPA1A; anti-HSP70 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification

• Applicable Applications for anti-HSP70 antibody: Western Blot (WB)
• Application Notes: WB: 1:2000-1:5000
• Immunogen: N term-peptide of human HSP70
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of COS1 celland HCT116 cells, using HSP70 antibody.)

Related Product Information for anti-HSP70 antibody

HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP-and co-chaperone-dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 (2, 3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1, 4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1, 3). They also play a role in vesicle formation and protein trafficking (2).

Product Categories/Family for anti-HSP70 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816. 2. Young, J.C. et al. (2003) Trends Biochem. Sci. 28, 541-547. 3. Pratt, W.B. and Toft, D.O. (2003) Exp. Biol. Med. 228, 111-133. 4. Hohfeld, J. et al. (2001) EMBO Rep. 2, 885-890.

NCBI and Uniprot Product Information

• NCBI GI #: 147744565
• NCBI GeneID: 3304
• UniProt Accession #: P08107; P0DMV9
• Molecular Weight: 70kDa
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 1B
• NCBI Official Symbol: HSPA1B
• NCBI Official Synonym Symbols: HSP70-2; HSP70.2; HSP70-1B
• NCBI Protein Information: heat shock 70 kDa protein 1B
• Protein Family: HSP70 co-chaperone

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]