Western Blot (WB)
(Western blot analysis of extracts from rat brain cells, using ANP32B antibody.)
Rabbit ANP32B Polyclonal Antibody | anti-ANP32B antibody
ANP32B Antibody
Gene Names
ANP32B; APRIL; SSP29; PHAPI2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ANP32B; Polyclonal Antibody; ANP32B Antibody; Acidic (leucine rich) nuclear phosphoprotein 32 family member B; Acidic leucine rich nuclear phosphoprotein 32 family member B; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic nuclear phosphoprotein 32 family member B; Acidic protein rich in leucines; AN32B_HUMAN; ANP 32B; APRIL; OTTHUMP0000006377; PAL 31; PAL31; PHAPI 2; PHAPI2; PHAPI2 protein; PHAPI2a; Proliferation related acidic leucine rich protein; Putative HLA-DR-associated protein I-2; Silver stainable protein SSP 29; Silver stainable protein SSP29; Silver-stainable protein SSP29; Ssp 29; Ssp29; anti-ANP32B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ANP32B antibody detects endogenous levels of total ANP32B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
251
Applicable Applications for anti-ANP32B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Accumulates in the nuclei at the S phase and Cytoplasm. Lacks a nuclear localization signal.
Tissue Specificity
Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
Predicted Cross Reactivity
Zebrafish, Bovine, Horse, Sheep, Dog
Similarity
Zebrafish (83%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from rat brain cells, using ANP32B antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain cells, using ANP32B antibody.)
Related Product Information for anti-ANP32B antibody
Function: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
Subunit Structure: Monomer. Interacts with histones H3 and H4.
Post-translational Modifications: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
Similarity: Histone binding is mediated by the concave surface of the LRR region. Belongs to the ANP32 family.
Subunit Structure: Monomer. Interacts with histones H3 and H4.
Post-translational Modifications: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
Similarity: Histone binding is mediated by the concave surface of the LRR region. Belongs to the ANP32 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 29 kD
Predicted: 29 kDa
Predicted: 29 kDa
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member B
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member B
NCBI Official Symbol
ANP32B
NCBI Official Synonym Symbols
APRIL; SSP29; PHAPI2
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member B
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member B
Protein Family
UniProt Gene Name
ANP32B
UniProt Synonym Gene Names
APRIL; PHAPI2; PHAPI2
Uniprot Description
Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
Research Articles on ANP32B
Similar Products
Product Notes
The ANP32B anp32b (Catalog #AAA9605186) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANP32B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ANP32B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ANP32B anp32b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANP32B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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