Western Blot (WB)
(Western blot analysis of His-Tag recombinant protein with E12-169 diluted at 1:10, 000 1) His-MBP recombinant protein 2) His-Tag transfected 293T)
His-Tag Monoclonal Antibody
His-Tag Mouse Monoclonal Antibody
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Synonyms
His-Tag; Monoclonal Antibody; His-Tag Mouse Monoclonal Antibody; anti-His-Tag antibody
Clonality
Monoclonal
Clone Number
169
Specificity
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-His-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
WB: 1:5,000-10,000
IP: 1:200
IF: 1:500
IP: 1:200
IF: 1:500
Preparation and Storage
Store at-20 degree C for 1 year
Western Blot (WB)
(Western blot analysis of His-Tag recombinant protein with E12-169 diluted at 1:10, 000 1) His-MBP recombinant protein 2) His-Tag transfected 293T)
Western Blot (WB)
(Western blot analysis of His-Tag recombinant protein with E12-169 diluted at 1:10, 000 1) His-MBP recombinant protein 2) His-Tag transfected 293T)
Related Product Information for anti-His-Tag antibody
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Similar Products
Product Notes
The His-Tag (Catalog #AAA8503844) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:5,000-10,000 IP: 1:200 IF: 1:500. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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