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Immunocytochemistry/Immunofluorescence (ICC/IF))
Eukaryotes Total Phospholipid Assay Kit
EZClick Total Phospholipid Assay Kit (Cell-Based)
Reactivity
Eukaryotes
Synonyms
Total Phospholipid; EZClick Total Phospholipid Assay Kit (Cell-Based); Total Phospholipid assay kit
Reactivity
Eukaryotes
Samples
Suspension or adherent cell cultures
Detection Method
Fluorescence: Ex/Em 494/521nm
Features & Benefits
Simple, fast, does not require lengthy incubation times
This assay provides a convenient and accurate procedure to measure totla phopholipid content in cell cultures using metabolic labeling techniques
This assay provides a convenient and accurate procedure to measure totla phopholipid content in cell cultures using metabolic labeling techniques
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Total Phospholipid assay kit
Phospholipids are major component of the bilayers of all plasma membranes. A single phospholipid molecule consists of a phosphate group on one ("head"), and two side-by-side chains of fatty acids that make up the "tails". The phosphate head groups can be modified with organic molecules such as Choline (Cho). Cho-containing phospholipids (Phosphatidylcholines; PC) are critical for structural membrane integrity, cellular metabolism and signaling either as individual molecules or precursors of secondary messengers. Changes in global synthesis of Cho-containing phospholipids are an essential parameter in analysis of cellular response to both, physiological and pathological conditions, environmental stress, or drug treatment. To date, phospholipids biochemistry, cell biology and metabolism remain obscure, due to limited methods for their direct cellular visualization. EZClick Global Phospholipid Synthesis Assay Kit offers a simple and robust method to label and visualize newly synthesized phospholipids in vivo. Based on the metabolic incorporation of the choline analogs directly into their structure, modified phospholipid molecules can be detected with high sensitivity and spatial resolution by click chemistry with azide-containing dyes (Ex/Em= 494/521 nm). This kit enables quantitative analyses of global biosynthesis/turnover of Cho-containing phospholipids in cells. Cells show strong incorporation of Cho analogs into all classes of phospholipids that can be assayed by microplate reader and fluorescence microscope. The kit provides sufficient materials for 100 assays.
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Product Notes
The Eukaryotes Total Phospholipid (Catalog #AAA849723) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849723 Assay Kit recognizes Eukaryotes Total Phospholipid. It is sometimes possible for the material contained within the vial of "Total Phospholipid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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