BRD3 bromodomain Recombinant Protein | BRD3 recombinant protein

BRD3 bromodomain (1-416 aa) (His-Tag), human recombinant

Cat. Num. AAA845310

• Gene Names: BRD3; ORFX; RING3L
• Applications: SDS-Page
• Purity: >=85%
• Synonyms: BRD3 bromodomain; BRD3 bromodomain (1-416 aa) (His-Tag); human recombinant; ORFX; RING3L; Bromodomain containing protein 3; RING3-like protein; BRD3 recombinant protein

• Host: E Coli
• Purity/Purification: >=85%
• Form/Format: 0.5 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Positions: 1-416
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSTATTV APAGIPATPG PVNPPPPEVS NPSKPGRKTN QLQYMQNVVV KTLWKHQFAW PFYQPVDAIK LNLPDYHKII KNPMDMGTIK KRLENNYYWS ASECMQDFNT MFTNCYIYNK PTDDIVLMAQ ALEKIFLQKV AQMPQEEVEL LPPAPKGKGR KPAAGAQSAG TQQVAAVSSV SPATPFQSVP PTVSQTPVIA ATPVPTITAN VTSVPVPPAA APPPPATPIV PVVPPTPPVV KKKGVKRKAD TTTPTTSAIT ASRSESPPPL SDPKQAKVVA RRESGGRPIK PPKKDLEDGE VPQHAGKKGK LSEHLRYCDS ILREMLSKKH AAYAWPFYKP VDAEALELHD YHDIIKHPMD LSTVKRKMDG REYPDAQGFA ADVRLMFSNC YKYNPPDHEV VAMARKLQDV FEMRFAKMP
• Sequence Length: 726

• Applicable Applications for BRD3 recombinant protein: SDS-PAGE
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for BRD3 recombinant protein

Background: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD3 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease. Recombinant human BRD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for BRD3 recombinant protein

Epigenetics; Reader Domains; Bromodomains; BromodomainsProteins and Enzymes; Other Proteins & EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 11067749
• NCBI GeneID: 8019
• NCBI Accession #: NP_031397.1
• NCBI GenBank Nucleotide #: NM_007371.3
• UniProt Accession #: Q15059; Q4G5Y3; Q5T1R7; Q8N5M3; Q92645; B1APD9
• Molecular Weight: 48.1 kDa (439 aa, 1-416 aa + His Tag), confirmed by MALDI-TOF.
• NCBI Official Full Name: bromodomain-containing protein 3
• NCBI Official Synonym Full Names: bromodomain containing 3
• NCBI Official Symbol: BRD3
• NCBI Official Synonym Symbols: ORFX; RING3L
• NCBI Protein Information: bromodomain-containing protein 3
• UniProt Protein Name: Bromodomain-containing protein 3
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD3
• UniProt Synonym Gene Names: KIAA0043; RING3L
• UniProt Entry Name: BRD3_HUMAN

NCBI Description

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; chromatin modification