DHFR active protein

Human Recombinant DHFR

Cat. Num. AAA844661

• Gene Names: DHFR; DYR; DHFRP1
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: DHFR; Human Recombinant DHFR; Dihydrofolate reductase.; DHFR active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 30% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
• Sequence Length: 187

• Applicable Applications for DHFR active protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 mug of protein (determined by LAL method)
• Biological Activity: Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction.
• Results: 1.5 - 2.5 units/ml
• Unit Definition: One unit will convert 1.0 umole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for DHFR active protein

Background: DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Human dihydrofolate reductase has been used in a study to investigate the stable expression of green fluorescent protein and the targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis. Human dihydrofolate reductase has also been used in a study to investigate the structural analysis of human dihydrofolate reductase as a binary complex.

Product Categories/Family for DHFR active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4503323
• NCBI GeneID: 1719
• NCBI Accession #: NP_000782.1
• NCBI GenBank Nucleotide #: NM_000791.3
• UniProt Accession #: P00374; Q14130; Q6IRW8; B4DDD2
• Molecular Weight: 23.6 kDa (207 aa, 1-187 aa + NT His-Tag)
• NCBI Official Full Name: dihydrofolate reductase isoform 1
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: DHFR
• NCBI Official Synonym Symbols: DYR; DHFRP1
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Viral dihydrofolate reductase
• UniProt Gene Name: DHFR
• UniProt Entry Name: DYR_HUMAN

NCBI Description

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Oxidoreductase; EC 1.5.1.3; Cofactor and Vitamin Metabolism - one carbon pool by folate

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: mRNA binding; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; vitamin metabolic process; one-carbon compound metabolic process; nitric oxide metabolic process; G1/S-specific transcription in mitotic cell cycle; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; mitotic cell cycle; response to methotrexate; folic acid metabolic process; regulation of nitric-oxide synthase activity; glycine biosynthetic process; water-soluble vitamin metabolic process; G1/S transition of mitotic cell cycle

Disease: Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency