Western Blot (WB)
(All lanes : Anti-UBA6 Antibody (C-term) at 1:2000 dilutionLane 1: Daudi whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: Hela whole cell lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit UBA6 Polyclonal Antibody | anti-UBA6 antibody
UBA6 Antibody (C-term)
Predicted: Dog
Predicted: Dog
Western Blot (WB)
(All lanes : Anti-UBA6 Antibody (C-term) at 1:2000 dilutionLane 1: Daudi whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: Hela whole cell lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-UBA6 Antibody (C-term) at 1:2000 dilutionLane 1: Daudi whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: Hela whole cell lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).
NCBI and Uniprot Product Information
NCBI Description
Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).[supplied by OMIM, Mar 2008]
Uniprot Description
UBE1L2: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. Belongs to the ubiquitin-activating E1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 4q13.2
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; FAT10 activating enzyme activity; ATP binding
Biological Process: ubiquitin-dependent protein catabolic process; protein ubiquitination
Research Articles on UBA6
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Product Notes
The UBA6 uba6 (Catalog #AAA9232185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBA6 Antibody (C-term) reacts with Human, Mouse Predicted: Dog and may cross-react with other species as described in the data sheet. AAA Biotech's UBA6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 0.25. Researchers should empirically determine the suitability of the UBA6 uba6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBA6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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