IL-17E active protein

Human CellExp IL-17E, human recombinant

Cat. Num. AAA844434

• Gene Names: IL25; IL17E
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: IL-17E; Human CellExp IL-17E; human recombinant; IL-25; Interleukin-25; IL-17E active protein

• Host: HEK 293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 177

• Applicable Applications for IL-17E active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug).
• Activity (Specifications/test method): The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
• Biological Activity: The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for IL-17E active protein

Background: Interleukin 25 (IL25) also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-kappaB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.

Product Categories/Family for IL-17E active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; InterleukinsCytokines; Growth Factors & Hormones; Cytokines & Growth Fact

NCBI and Uniprot Product Information

• NCBI GI #: 12232485
• NCBI GeneID: 64806
• NCBI Accession #: NP_073626.1
• NCBI GenBank Nucleotide #: NM_022789.3
• UniProt Accession #: Q9H293; Q2M3F0; Q8IZV3; Q8WXB0
• Molecular Weight: This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 16.7 kDa. The predicted N-terminus is Tyr 33. DTT-reduced Protein migrates as 24 kDa, 27 kDa and 31 kDa due to different glycosylation.
• NCBI Official Full Name: interleukin-25 isoform 1
• NCBI Official Synonym Full Names: interleukin 25
• NCBI Official Symbol: IL25
• NCBI Official Synonym Symbols: IL17E
• NCBI Protein Information: interleukin-25
• UniProt Protein Name: Interleukin-25
• UniProt Gene Name: IL25
• UniProt Synonym Gene Names: IL17E; IL-25; IL-17E
• UniProt Entry Name: IL25_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter