SIRT6 active protein

Active SIRT6 (GST-tagged), human recombinant

Cat. Num. AAA844343

• Gene Names: SIRT6; SIR2L6
• Applications: SDS-Page
• Purity: >=85%
• Synonyms: SIRT6; Active SIRT6 (GST-tagged); human recombinant; NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6; SIRT6 active protein

• Host: E Coli
• Purity/Purification: >=85%
• Form/Format: Human SIRT6 protein is a solution of 1 mg/ml in 50 mM Tris, 100 mM NaCl, pH 8.0 containing 20% glycerol. ; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 328

• Applicable Applications for SIRT6 active protein: SDS-PAGE; HPLC
• Activity (Specifications/test method): MyBioSource's Active GST-tagged SIRT6 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >3 mU/mg (One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 umol of AFC/min at 37 degree C).
• Biological Activity: >3 mU/mg
• Results: >3 mU/mg
• Unit Definition: One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 umol of AFC/min at 37 degree C
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for SIRT6 active protein

Background: Human Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards Lys-9 and Lys-56 of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-alpha.

Product Categories/Family for SIRT6 active protein

Epigenetics; Eraser Enzymes; Sirtuins (SIRTs); EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 300797597
• NCBI GeneID: 51548
• NCBI Accession #: NP_001180214.1
• NCBI GenBank Nucleotide #: NM_001193285.1
• UniProt Accession #: Q8N6T7; O75291; Q6IAF5; Q6PK99; Q8NCD2; Q9BSI5; Q9BWP3; Q9NRC7; Q9UQD1; B2RCD0
• Molecular Weight: Human SIRT6 is a 65.3 kDa (1-355 aa + N-terminal GST tag) protein. It runs as ~62 kDa on a SDS-PAGE.
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-6 isoform 2
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: SIRT6
• NCBI Official Synonym Symbols: SIR2L6
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT6
• UniProt Synonym Gene Names: SIR2L6
• UniProt Entry Name: SIR6_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.31; Deacetylase; EC 3.5.1.-; Transferase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear telomeric heterochromatin; nucleolus; nucleus

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; histone deacetylation