Western Blot (WB)
(SIRT6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SIRT6 antibody detected the SIRT6 protein (arrow).)
Rabbit anti-Human SIRT6 Polyclonal Antibody | anti-SIRT6 antibody
SIRT6, CT (SIRT6, SIR2L6, NAD-dependent protein deacetylase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2-like protein 6)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(SIRT6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SIRT6 antibody detected the SIRT6 protein (arrow).)
Western Blot (WB)
(SIRT6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SIRT6 antibody detected the SIRT6 protein (arrow).)
Immunohistochemistry (IHC)
(SIRT6 Antibody (C-term) (AP14024b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIRT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SIRT6 Antibody (C-term) (AP14024b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIRT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]
Uniprot Description
Function: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span
By similarity. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD+ in the absence of acetylated substrate. Ref.8 Ref.11 Ref.12 Ref.13 Ref.15
Catalytic activity: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. Ref.8
Cofactor: Binds 1 zinc ion per subunit
By similarity.
Subunit structure: Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Ref.11 Ref.13
Subcellular location: Nucleus › nucleoplasm. Note: Predominantly nuclear. Associated with telomeric heterochromatin regions. Ref.7 Ref.8 Ref.11
Miscellaneous: The reported ADP-ribosyltransferase activity of sirtuins is likely some inefficient side reaction of the deacetylase activity and may not be physiologically relevant
By similarity.
Sequence similarities: Belongs to the sirtuin family. Class IV subfamily.Contains 1 deacetylase sirtuin-type domain.
Sequence caution: The sequence AAC34468.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAD15478.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAH04218.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CCDS.
Research Articles on SIRT6
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Product Notes
The SIRT6 sirt6 (Catalog #AAA647110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT6, CT (SIRT6, SIR2L6, NAD-dependent protein deacetylase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2-like protein 6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the SIRT6 sirt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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