Apolipoprotein AIV Protein | ApoA4 protein

Apolipoprotein AIV, Human Plasma

Cat. Num. AAA844006

• Applications: SDS-Page
• Purity: >= 85% by SDS-PAGE
• Synonyms: Apolipoprotein AIV; Human Plasma; Apolipoprotein A-IV; apo AIV; Apo-AIV; ApoA-IV; ApoA4 protein

• Purity/Purification: >= 85% by SDS-PAGE
• Form/Format: Form: Frozen; Fomulation: Frozen in 10 mM NH₄HCO₃, pH 8.2
• Sequence Length: 396

• Applicable Applications for ApoA4 protein: SDS-PAGE
• Source: Human Plasma. Prepared from plasma shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
• Extinction Coefficient: 1.00
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Aliquot and store at -70 degree C or lower. Avoid repeated freezing and thawing cycles.; Shipping: Ships with Dry Ice.

Testing Data

(Apolipoprotein AIV, Human Plasma 4-12% Bis-Tris-HCI NuPAGE gel1. MW Standard2. Apo AIV - 5 ug (reduced / heated)3. Apo AIV - 10 ug (reduced / heated)4. Apo AIV - 5 ug (non-reduced / no heat)5. Apo AIV - 10 ug (non-reduced / no heat))

Related Product Information for ApoA4 protein

Background: Apolipoprotein AIV (Apo AIV) is a glycoprotein synthesized by the human intestine. Synthesis appears to be stimulated by the absorption of high density lipoproteins that are rich in triglycerides rather than by the uptake or re-esterification of fatty acids. The formation of chylomicrons acts as a signal for the induction of Apo AIV synthesis a signal enhanced by a factor from the ileum, probably PYY. Apo AIV alters the activity of the key enzymes (LPL and LCAT) of lipoprotein metabolism and cholesterol efflux from extra hepatic tissues. Also, since stimulation of intestinal synthesis and secretion by lipid absorption are rapid, Apo AIV likely plays a role in the short-term regulation of food intake. Other evidence suggests involvement in the long-term regulation of both food intake and body weight. Chronic ingestion of high-fat diet blunts the intestinal Apo AIV response to lipid feeding and, consequently, may explain why the chronic ingestion of a high-fat diet predisposes both animals and humans to obesity. It is required for efficient activation of lipoprotein lipase by ApoC-II and is a potent activator of LCAT. Apo AIV is an amphipathic protein that can emulsify lipids and has been linked to protective roles against cardiovascular disease and obesity.

Product Categories/Family for ApoA4 protein

Proteins and Enzymes; Native Proteins Diabetes; Obesity & Metabolic Syndrome; Lipids Metabolism; Proteins/Enzymes; ApolipoproteinsCell Damage & Oxidative Stress; EnzymesProteins and Enzymes; ApolipoproteinsProteins and Enzymes; Proteins and Enzymes (A-Z

NCBI and Uniprot Product Information

• NCBI GI #: 93163358
• NCBI GeneID: 337
• UniProt Accession #: P06727; Q14CW8; Q6Q787; A8MSL6
• Molecular Weight: 46 kDa
• NCBI Official Full Name: Apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Endoplasmic reticulum; Lipid-binding; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: antioxidant activity; lipid transporter activity; protein binding; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: multicellular organismal lipid catabolic process; phototransduction, visible light; cholesterol metabolic process; positive regulation of fatty acid biosynthetic process; removal of superoxide radicals; protein-lipid complex assembly; lipid homeostasis; neurite regeneration; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; leukocyte adhesion; reverse cholesterol transport; phospholipid efflux; innate immune response in mucosa; regulation of cholesterol absorption; hydrogen peroxide catabolic process; response to lipid hydroperoxide; positive regulation of lipoprotein lipase activity; retinoid metabolic process; regulation of cholesterol transport