Rabbit anti-Human, Mouse Cadherin Polyclonal Antibody | anti-Cdh1 antibody

Cadherin Antibody

Cat. Num. AAA841336

• Gene Names: Cdh1; Um; UVO; Ecad; E-cad; L-CAM; AA960649
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Cadherin; Polyclonal Antibody; Cadherin Antibody; P-cadherin; N-Cadherin; E-Cadherin; K-Cadherin; M-jadherin; R-Cadherin; anti-Cdh1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-cadherin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 884

• Applicable Applications for anti-Cdh1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Western blotting (1-2 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects E- and P-cadherin (~95 kDa). Jurkat cell lysate can be used as a positive control.
• Immunogen: Synthetic peptide surrounding amino acid 388 of human Cadherin
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Cdh1 antibody

Background: Cadherins comprise a family of Ca-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of cadherins is thomugh to be responsible for binding specificity, transmembrane domains and carboxy terminal domains. The relative short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function.

NCBI and Uniprot Product Information

• NCBI GI #: 6753374
• NCBI GeneID: 12550
• NCBI Accession #: NP_033994.1
• NCBI GenBank Nucleotide #: NM_009864.2
• UniProt Accession #: P09803; Q61377
• Molecular Weight: 98,256 Da
• NCBI Official Full Name: cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Official Synonym Symbols: Um; UVO; Ecad; E-cad; L-CAM; AA960649
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_MOUSE

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Motility/polarity/chemotaxis; Ubiquitin ligase; Cell adhesion; Membrane protein, integral

Cellular Component: apical junction complex; Golgi apparatus; internal side of plasma membrane; cell surface; focal adhesion; basolateral plasma membrane; lateral loop; integral to membrane; trans-Golgi network; catenin complex; intercellular junction; actin cytoskeleton; adherens junction; cell-cell adherens junction; membrane; axon; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; nerve terminal; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; metal ion binding; gamma-catenin binding; beta-catenin binding; GTPase activating protein binding; cell adhesion molecule binding; calcium ion binding; ankyrin binding; glycoprotein binding; protein phosphatase binding

Biological Process: in utero embryonic development; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of epithelial cell proliferation; trophectodermal cell differentiation; regulation of water loss via skin; cell-cell adhesion; sensory perception of sound; regulation of protein localization; synaptogenesis; calcium-dependent cell-cell adhesion; protein metabolic process; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; cell adhesion; homophilic cell adhesion; protein homooligomerization; negative regulation of epithelial cell proliferation